NFE2L2 antibody (10R-1495)

Mouse monoclonal NFE2L2 antibody

Synonyms Monoclonal NFE2L2 antibody, Anti-NFE2L2 antibody, NFEL 2, NFEL-2 antibody, NFE2L2, Nuclear factor (erythroid -derived 2) like 2 antibody, NFEL-2, NFEL 2 antibody, NRF2 antibody
Specificity Human NFE2L2
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen NFE2L2 antibody was raised in mouse using recombinant Human Nuclear Factor (Erythroid-Derived 2)-Like 2

Images

Immunofluorescent staining using NFE2L2 antibody (10R-1495)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NFE2L2 antibody.

Specifications

Host Mouse
Clone 437C2a
Isotype IgG1
Method of Purification NFE2L2 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer NFE2L2 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2, is a transcription factor that in humans is encoded by the NFE2L2 gene. Nrf2 is a master regulator of the antioxidant response. The antioxidant response is important for the amelioration of oxidative stress.

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Images

  • Immunofluorescent staining using NFE2L2 antibody (10R-1495) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NFE2L2 antibody.
  • Western Blot analysis of immunized recombinant protein using NFE2L2 antibody (10R-1495)

Availability: In stock

Price: $190.00
Size: 50 ug
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