NFKB C-rel antibody (20-CR07)

Rabbit polyclonal NFKB C-rel antibody

Synonyms Polyclonal NFKB C-rel antibody, Anti-NFKB C-rel antibody, NFkappaB antibody, Nuclear factor NF-kappa-B p65 subunit antibody, NFKB antibody, nfkb antibody, NF-kB antibody, NF-kappaB antibody
Specificity Human NFKB C-rel
Cross Reactivity To be determined by end-user
Applications ELISA, WB
Immunogen NFKB C-rel antibody was raised in rabbit using NFkB cRel peptide corresponding to a region near the C-terminus of the human protein conjugated to KLH as the immunogen.

Images

Western Blot analysis using NFKB C-rel antibody (20-CR07)

Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0. 1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1, 000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 mins each. The western blot was incubated with Goat anti-Rabbit IgG (H + L) (HRP) diluted 1:2, 000 for 1 h. Washes with TBS preceded color development.

Specifications

Host Rabbit
Method of Purification NFKB C-rel antibody was purified by delipidation and defibrination.
Form & Buffer 0.02M K3PO4,pH 7.2, with 0.15M NaCl, and 0.01%NaN3.
Concentration 80.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:5,000-1:25,000, WB: 1:500-1:3,000

Storage & Safety

Storage Store at -20 deg C. Aliquot and freeze at -20 deg C or below for long term storage. Avoid repeated freeze/thaw cycles.
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

General Information

Biological Significance NFkappaB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NF kb1) subunits.

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Images

  • Western Blot analysis using NFKB C-rel antibody (20-CR07) | Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0. 1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1, 000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5 mins each. The western blot was incubated with Goat anti-Rabbit IgG (H + L) (HRP) diluted 1:2, 000 for 1 h. Washes with TBS preceded color development.

Availability: In stock

Price: $575.00
Size: 100 ul
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