NFKBIA protein (His tag) (80R-2014)

Recombinant human NFKBIA protein (His tag)

Synonyms IKBA protein, MAD-3 protein, NFKBI protein, NF-kappa-B inhibitor alpha protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of NFKBIA protein (His tag) (80R-2014)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-317 amino acids: MGSSHHHHHH SSGLVPRGSH MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL
Expression System E.coli
Purity > 90% pure
Molecular Weight 37.7 kDa (337aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations NFKBIA protein (His tag) has been used in SDS-PAGE, and may be suitable for use in other applications, to be determined by the end user.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance NF-kappa-B inhibitor alpha, also known as NFKBIA, is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon). NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. Upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Recombinant human NFKBIA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of NFKBIA protein (His tag) (80R-2014) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $395.00
Size: 50 ug
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