NFYB antibody (10R-1252)

Mouse monoclonal NFYB antibody

Synonyms Monoclonal NFYB antibody, Anti-NFYB antibody, NF-YB antibody, NF-YB antibody, HAP3 antibody, HAP3 antibody, CBF-B antibody, CBF-B antibody, CBF-A antibody, Nuclear transcription factor Y subunit beta antibody, Nuclear transcription factor Y subunit beta antibody, NFYB antibody, Anti NFYB antibody, CBF-A antibody
Specificity Human NFYB
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC
Immunogen NFYB antibody was raised in mouse using recombinant Nuclear Transcription Factor Y, Beta (Nfyb)

Images

Immunofluorescent staining using NFYB antibody (10R-1252)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NFYB antibody.

Specifications

Host Mouse
Clone NFYBA7
Isotype IgG1
Method of Purification NFYB antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

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Images

  • Immunofluorescent staining using NFYB antibody (10R-1252) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NFYB antibody.
  • Western Blot analysis of immunized recombinant protein using NFYB antibody (10R-1252) | Left: NFYB staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
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