NHP2L1 protein (His tag) (80R-1573)
Purified recombinant Human NHP2L1 protein
|Synonyms||NHPL1-2 protein, OTK27 protein, NHPX protein, FA1 protein, NHPL1 2, SNRNP15-5 protein, NHP2L1, NHPL1 2 protein, SPAG12 protein, NHP2(non-histone chromosome protein 2)-like 1 protein, SSFA1, SNU13 protein, NHPL1-2|
Coomassie Blue stained SDS-PAGE of NHP2L1 protein (His tag) (80R-1573)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-128 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV|
|Purity||> 95% pure|
|Molecular Weight||16.7 kDa(152aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, containing 100mM NaCl and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NHP2L1 belongings to the ribosomal protein L7Ae family. This protein localizes to the nucleus, mainly concentrated in the dense fibrillar component of the nucleolus. It directly binds to the 5' stem-loop of U4 snRNA and plays an important role in the late stage of spliceosome assembly, prior to step I of splicing catalysis. Recombinant human NHP2L1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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