Nicastrin antibody (10R-1141)

Mouse monoclonal Nicastrin antibody

Synonyms Monoclonal Nicastrin antibody, Anti-Nicastrin antibody, APH2 antibody, APH2 antibody, Nicastrin antibody, KIAA0253 antibody, Anti Nicastrin antibody , nicastrin precursor antibody, KIAA0253 antibody, nicastrin antibody, nicastrin antibody, nicastrin precursor antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, WB
Immunogen Nicastrin antibody was raised in mouse using recombinant human Nicastrin (34-153aa) purified from E. coli as the immunogen.

Images

Western Blot analysis of Nicastrin antibody (10R-1141)

The extracts of Mouse brain (80 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Nicastrin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT3B2
Isotype IgG2b kappa
Source Anti-human Nicastrin antibody, clone AT3B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nicastrin protein.
Method of Purification Nicastrin antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1:500-1:1,000

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Nicastrin is a large protein that is a component of an enzyme called gamma-secretase, which is lodged in the cell's membrane. Nicastrin protein is physically associated with presenilin and plays an important role in stabilization and correct localization of presenilin to the membrane-bound gamma-secretase complex. Nicastrin also serves as a docking site for gamma-secretase substrates such as APP and Notch, directly binding to them and presenting them properly to gamma-secretase to ensure the correct cleavage process

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Images

  • Western Blot analysis of Nicastrin antibody (10R-1141) | The extracts of Mouse brain (80 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human Nicastrin (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $265.00
Size: 100 ul
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