NME1 antibody (10R-1852)

Mouse monoclonal NME1 antibody

Synonyms Monoclonal NME1 antibody, Anti-NME1 antibody, NME-1 antibody, NM23-H1 antibody, NME 1 antibody, NME 1 antibody, NME-1, Nucleoside diphosphate kinase A antibody, NME1, NME-1 antibody, NM23 long variant antibody, NME 1, NDP kinase A antibody
Cross Reactivity Human
Applications ELISA, IF, WB
Immunogen NME1 antibody was raised in Mouse using a purified recombinant fragment of human NME1 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using NME1 antibody (10R-1852) (NME1 antibody)

FC analysis of Jurkat cells using NME1 antibody (green) and negative control (purple).

Specifications

Host Mouse
Clone 4B2
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NME1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Flow Cytometric assay using Western Blot analysis using NME1 antibody (10R-1852) (NME1 antibody) | FC analysis of Jurkat cells using NME1 antibody (green) and negative control (purple).
  • Western Blot analysis using NME1 antibody (10R-1852) | Western Blot showing NME1 antibody used against NME1 (AA: 27-177)-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemical staining using NME1 antibody (10R-1852) | Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 antibody
  • Immunofluorescent staining using Western Blot analysis using NME1 antibody (10R-1852) (NME1 antibody) | Immunofluorescence analysis of Hela cells using NME1 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Availability: In stock

Price: $345.00
Size: 100 ul
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