NPT antibody (10R-1921)
Mouse monoclonal NPT antibody
|Synonyms||Monoclonal NPT antibody, Anti-NPT antibody, NPT antibody, NptII antibody, NptII antibody, Neomycin phosphotransferase II antibody, Neomycin phosphotransferase II antibody, Anti NPT antibody|
|Immunogen||NPT antibody was raised in Mouse using a purified recombinant fragment of NPT expressed in E. coli as the immunogen.|
Western Blot analysis using NPT antibody (10R-1921)
Western Blot showing NPT antibody used against truncated NPT recombinant protein.
|Form & Buffer||Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:10,000, WB: 1:500-1:2000|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NPT is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPT is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.|
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