NPTII antibody (20C-CR1112RP)

Rabbit polyclonal NPTII antibody

Synonyms Polyclonal NPTII antibody, Anti-NPTII antibody, Neomycin Phosphotransferase II antibody
Specificity Human NPTII
Cross Reactivity To be determined by end-user
Applications ELISA, WB
Immunogen NPTII antibody was raised in rabbit using purified neomycin phosphotransferase II as the immunogen.

Specifications

Host Rabbit
Isotype IgG
Form & Buffer Supplied as an IgG fraction in PBS, pH 7.4 with 0.05% NaN3
Concentration 5 mg/ml

Usage & Assay Information

Usage Recommendations Use at starting dilution 1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -80 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

References
Add a Paper

Zheng Qing Fu, Ming Guo, James R. Alfano

Pseudomonas syringae HrpJ Is a Type III Secreted Protein That Is Required for Plant Pathogenesis, Injection of Effectors, and Secretion of the HrpZ1 Harpin

Journal of Bacteriology

Volume: 188 Issue: 17 Page: 6060-6069 DOI: 10.1128/JB.00718-06

Schmidt FI, Hanke L, Morin B, Brewer R, Brusic V, Whelan SP, Ploegh HL

Phenotypic lentivirus screens to identify functional single domain antibodies

Nat Microbiol.

Issue: 1 (2016) PMID: 27573105 DOI: 10.1038/nmicrobiol.2016.80

Reviews

You need to be logged in to write a review. Please login here
Submitted By
Date
Application product used in Western Blotting
Usage 1:1000 in 3% milk for 1 hour
Comments Excellent antibody, specific and clear, one of the few available ones that works!

Samples were isolated from plant extract from Arabidopsis seedlings. 100ug protein was loaded onto the gel and transferred to a PVDF membrane for blotting. NPTII antibody was dluted 1:1000 in 3% milk and allowed to incubate for 1 hour.

Antibody works with high specificity on multiple samples containing crude extract of transgenic Arabidopsis thaliana. We have used the following lines expressing E. coli NPTII to test antibody specificity: ku70 (Riha et al. (2002)), prt6-2 (SALK_051088, aka prt6-5 in Graciet et al. (2009), and prt6-1 in Holman et al. (2009)), and mpk1 (Bartels et al. (2009)). A plant sensitive for kanamycin was used as negative control.
Rating 5

Availability: In stock

Price: $165.00
Size: 1 mg
OR
Shipping
View Our Distributors