NRK1 protein (His tag) (80R-2310)
Purified recombinant Human NRK1 Protein (His tag)
|Synonyms||NRK1, NRK 1, RP11-235O14.2 protein, NRK-1, bA235O14.2 protein, NRK 1 protein, NRK1 protein, NRK-1 protein|
Coomassie Blue stained SDS-PAGE of NRK1 protein (His tag) (80R-2310)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MGSSHHHHHH SSGLVPRGSH MGSMKTFIIG ISGVTNSGKT TLAKNLQKHL PNCSVISQDD FFKPESEIET DKNGFLQYDV LEALNMEKMM SAISCWMESA RHSVVSTDQE SAEEIPILII EGFLLFNYKP LDTIWNRSYF LTIPYEECKR RRSTRVYQPP DSPGYFDGHV WPMYLKYRQE MQDITWEVVY LDGTKSEEDL FLQVYEDLIQ ELAKQKCLQV TA|
|Purity||> 90% pure|
|Molecular Weight||25.6 kDa|
|Form & Buffer||Supplied in liquid form in 20 mM Tris-HCl buffer, pH8.0, 10% glycerol, 2mM DTT, 200mM NaCl|
Storage & Safety
|Storage||Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||NRK1 belongs to the uridine kinase family. NAD+ is essential for life in all organisms, both as a coenzyme for oxidoreductases and as a source of ADPribosyl groups used in various reactions, including those that retard aging in experimental systems. Nicotinic acid and nicotinamide were defined as the vitamin precursors of NAD+. This enzyme catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside to form nicotinamide mononucleotide and nicotinic acid mononucleotide . The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine. Recombinant human NRK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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