NT5M protein (His tag) (80R-1925)

Purified recombinant NT5M protein

Synonyms NTM 5, NTM 5 protein, NTM-5 protein, mitochondrial protein, NTM-5, NT5M, 5? (3?)-deoxyribonucleotidase protein, DNT2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information NT5M protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Images

Coomassie Blue stained SDS-PAGE of NT5M protein (His tag) (80R-1925)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 32-228 amino acids: MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC
Expression System E.coli
Purity > 95% pure
Molecular Weight 25.1 kDa
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, with 20% glycerol, 0.1M NaCl and 1mM DTT.
Concentration 0.5 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C or -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance NT5M, also known as DNT2, belongs to the 5'(3')-deoxyribonucleotidase family. It localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unknown etiology. Recombinant human NT5M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of NT5M protein (His tag) (80R-1925) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 10 ug
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