Nucleolin antibody (70R-1328)

Rabbit polyclonal Nucleolin antibody raised against the C terminal of NCL

Synonyms Polyclonal Nucleolin antibody, Anti-Nucleolin antibody, NCL antibody
Specificity Nucleolin antibody was raised against the C terminal of NCL
Cross Reactivity Human,Rat,Dog
Applications IHC, WB
Immunogen Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Assay Information Nucleolin Blocking Peptide, catalog no. 33R-3278, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Images

Western Blot analysis using Nucleolin antibody (70R-1328)

Nucleolin antibody (70R-1328) used at 1.25 ug/ml to detect target protein.

Specifications

Host Rabbit
Method of Purification Total IgG Protein A purified
Molecular Weight 78 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 100ul distilled water for a 1mg/ml concentration of NCL antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 1.25 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

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Images

  • Western Blot analysis using Nucleolin antibody (70R-1328) | Nucleolin antibody (70R-1328) used at 1.25 ug/ml to detect target protein.

Availability: In stock

Price: $275.00
Size: 100 ug
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