Nucleolin antibody (70R-4742)

Rabbit polyclonal Nucleolin antibody raised against the N terminal of NCL

Synonyms Polyclonal Nucleolin antibody, Anti-Nucleolin antibody, NCL antibody
Specificity Nucleolin antibody was raised against the N terminal of NCL
Cross Reactivity Human
Applications IHC, WB
Immunogen Nucleolin antibody was raised using the N terminal of NCL corresponding to a region with amino acids GKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDED
Assay Information Nucleolin Blocking Peptide, catalog no. 33R-3357, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Images

Immunohistochemical staining using Nucleolin antibody (70R-4742)

NCL antibody staining. Paraffin Embedded Tissue: Human Lung Cellular Data: Alveolar cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X

Specifications

Host Rabbit
Method of Purification Affinity purified
Molecular Weight 78 kDa (MW of target protein)
Form & Buffer Lyophilized powder. Add 50ul distilled water for a 1mg/ml concentration of NCL antibody in PBS
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations WB: 0.5 ug/ml; IHC: 4-8 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles.

General Information

Biological Significance Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb.

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Images

  • Immunohistochemical staining using Nucleolin antibody (70R-4742) | NCL antibody staining. Paraffin Embedded Tissue: Human Lung Cellular Data: Alveolar cells Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X
  • Immunohistochemical staining using Nucleolin antibody (70R-4742) | NCL antibody staining. Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X
  • Immunohistochemical staining using Nucleolin antibody (70R-4742) | Human Skin
  • Western blot analysis using Nucleolin antibody (70R-4742) | Recommended NCL Antibody Titration: 0.2-1 ug/ml

Availability: In stock

Price: $345.00
Size: 50 ug
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