Nucleophosmin antibody (10R-1959)

Mouse monoclonal Nucleophosmin antibody

Synonyms Monoclonal Nucleophosmin antibody, Anti-Nucleophosmin antibody, MGC104254 antibody, nucleolar phosphoprotein B23 antibody, NPM antibody, nucleophosmin/nucleoplasmin family antibody, Nucleophosmin antibody, numatrin antibody, B23 antibody, nucleolar phosphoprotein B23 antibody, NPM antibody, numatrin antibody, MGC104254 antibody, NPM antibody, B23 antibody, Anti Nucleophosmin antibody, numatrin antibody, member 1 antibody, Nucleolar phosphoprotein B23 antibody, Nucleolar phosphoprotein B23 antibody, nucleophosmin/nucleoplasmin family antibody, member 1 antibody, numatrin antibody, NPM antibody
Cross Reactivity Human, Monkey
Applications ELISA, IF, IHC, WB
Immunogen Nucleophosmin antibody was raised in Mouse using a purified recombinant fragment of human NPM expressed in E. coli as the immunogen.

Images

Immunofluorescent staining using Western Blot analysis using Nucleophosmin antibody (10R-1959) (Nucleophosmin antibody)

Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Specifications

Host Mouse
Clone 7H10B9
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IF: 1:200-1:1000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Nucleophosmin is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.

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Images

  • Immunofluorescent staining using Western Blot analysis using Nucleophosmin antibody (10R-1959) (Nucleophosmin antibody) | Confocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
  • Western Blot analysis using Nucleophosmin antibody (10R-1959) | Western Blot showing NPM antibody used against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.
  • Immunohistochemical staining using Nucleophosmin antibody (10R-1959) | Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissues, showing nuclear localization using NPM antibody with DAB staining.

Availability: In stock

Price: $295.00
Size: 100 ul
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