NUDT10 protein (His tag) (80R-1786)
Purified recombinant Human NUDT10 protein
|Synonyms||APS2 protein, NUDT 10, NUDT 10 protein, DIPP3a protein, NUDT-10, NUDT10, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha protein, hDIPP3alpha protein, NUDT-10 protein|
Coomassie Blue stained SDS-PAGE of NUDT10 protein (His tag) (80R-1786)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-164 amino acids: MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG GMEPEEEPGG AAVREVYEEA GVKGKLGRLL GVFEQNQDPK HRTYVYVLTV TELLEDWEDS VSIGRKREWF KVEDAIKVLQ CHKPVHAEYL EKLKLGGSPT NGNSMAPSSP DSDPLEHHHH HH|
|Purity||> 95% pure|
|Molecular Weight||19.5 kDa (172aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUDT10, also known as APS2 or DIPP3 alpha, is a member of the Nudix hydrolase family of pyrophosphatases. Nudix hydrolases contain a characteristic Nudix domain and are responsible for catalyzing the hydrolysis of nucleoside diphosphate derivatives. It functions as a manganese-dependent polyphosphate phosphohydrolase with an optimum pH of 8.5. This protein specifically metabolizes diadendosine-polyphosphates and, to a lesser extent, diphosphoinositol polyphosphates. Recombinant human NUDT10 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.|
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