NUDT14 protein (His tag) (80R-2669)
Purified recombinant NUDT14 protein (His tag)
|Synonyms||Uridine diphosphate glucose pyrophosphatase protein, UGPP protein, UGPPase protein, NUDT14, NUDT 14, NUDT-14, NUDT 14 protein, NUDT-14 protein|
SDS-PAGE analysis of NUDT14 protein (His tag) (80R-2669)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-222 aa: MGSSHHHHHH SSGLVPRGSH MGSMERIEGA SVGRCAASPY LRPLTLHYRQ NGAQKSWDFM KTHDSVTVLL FNSSRRSLVL VKQFRPAVYA GEVERRFPGS LAAVDQDGPR ELQPALPGSA GVTVELCAGL VDQPGLSLEE VACKEAWEEC GYHLAPSDLR RVATYWSGVG LTGSRQTMFY TEVTDAQRSG PGGGLVEEGE LIEVVHLPLE GAQAFADDPD IPKTLGVIFG VSWFLSQVAP NLDLQ|
|Purity||> 95% pure|
|Molecular Weight||26.5 kDa|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT|
Storage & Safety
|Storage||Can be stored at 4 deg C for short terms (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles|
|Biological Significance||Uridine diphosphate glucose pyrophosphatase, also known as NUDT14, is a 222 amino acid cytoplasmic protein that contains one nudix hydrolase domain and belongs to the nudix hydrolase family. NUDT14 hydrolyzes ADP-ribose into ribose 5-phosphate and AMP, and UDP-glucose to glucose 1-phosphate and UMP. Existing as a homodimer, NUDT14 binds magnesium as a cofactor and is encoded by a gene located on human chromosome 14. Recombinant human NUDT14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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