NUDT16 protein (His tag) (80R-1627)
Purified recombinant Human NUDT16 protein
|Synonyms||NUDT16, Nudix type motif 16 protein, NUDT-16 protein, NUDT 16, NUDT 16 protein, NUDT-16|
Coomassie Blue stained SDS-PAGE of NUDT16 protein (His tag) (80R-1627)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-195 amino acids: MGSSHHHHHH SSGLVPRGSH MAGARRLELG EALALGSGWR HACHALLYAP DPGMLFGRIP LRYAILMQMR FDGRLGFPGG FVDTQDRSLE DGLNRELREE LGEAAAAFRV ERTDYRSSHV GSGPRVVAHF YAKRLTLEEL LAVEAGATRA KDHGLEVLGL VRVPLYTLRD GVGGLPTFLE NSFIGSAREQ LLEALQDLGL LQSGSISGLK IPAHH|
|Purity||> 95% pure|
|Molecular Weight||23.4 kDa (215aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol, 1mM DTT and 100mM NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUDT16 is a nuclear nucleoside diphosphatase protein localized within foci in the nucleolus and nucleoplasm. This is a RNA-decapping enzyme that binds specifically to U8 snoRNA. It can remove m7G and m227G caps from RNAs, rendering them substrates for 5’-3’ exonucleases for degradation in vivo. Recombinant human NUDT16 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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