NUDT21 antibody (10R-1375)

Mouse monoclonal NUDT21 antibody

Synonyms Monoclonal NUDT21 antibody, Anti-NUDT21 antibody, NUDT-21, NUDT 21, DKFZp686H1588 antibody, NUDT-21 antibody, CPSF5 antibody, NUDT 21 antibody, CFIM25 antibody, Cleavage and polyadenylation specificity factor subunit 5 antibody, NUDT21
Specificity Human NUDT21
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, ICC, IP, WB
Immunogen NUDT21 antibody was raised in mouse using recombinant Human Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 21 (Nudt21)

Images

Flow Cytometric assay using NUDT21 antibody (10R-1375)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-NUDT21 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2203C3
Isotype IgG1
Method of Purification NUDT21 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer NUDT21 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, IP: 100-500 ug/sample, FC: 0.5-2 ug/sample, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

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Images

  • Flow Cytometric assay using NUDT21 antibody (10R-1375) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-NUDT21 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using NUDT21 antibody (10R-1375)
  • Immunofluorescent staining using NUDT21 antibody (10R-1375) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.01% Triton-X100 in PBS. The cells were immunostained with anti-NUDT21 antibody.

Availability: In stock

Price: $190.00
Size: 50 ug
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