NUDT21 protein (His tag) (80R-1693)
Purified recombinant Human NUDT21 protein
|Synonyms||CFIM25 protein, NUDT-21, NUDT21, NUDT 21 protein, NUDT 21, DKFZp686H1588, CPSF5 protein, Nudix (nucleoside diphosphate linked moiety X)-type motif 21 protein, NUDT-21 protein|
Coomassie Blue stained SDS-PAGE of NUDT21 protein (His tag) (80R-1693)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-227 amino acids: MGSSHHHHHH SSGLVPRGSH MSVVPPNRSQ TGWPRGVTQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN|
|Purity||> 90% pure|
|Molecular Weight||28.3 kDa (247aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 2mM DTT and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||NUDT21, also known as CPSF5 or CFIm25, is a member of the Nudix hydrolase family of pyrophosphatases. This protein localizes to the paraspeckles and forms a heterodimer with CPSF6 or CPSF7 to comprise the CFIm (mammalian cleavage factor I) complex. NUDT21 is the smaller subunit of the complex and is present in all heterodimer combinations. NUDT21 plays an important role in pre-mRNA 3' cleavage and polyadenylation processing. Recombinant human NUDT21 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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