O4 antibody (10R-8060)

Mouse monoclonal O4 antibody

Synonyms Monoclonal O4 antibody, Anti-O4 antibody, Oligodendrocyte Marker O4 antibody, Oligodendrocyte O4 antibody, Oligodendrocyte Marker O4, O4
Specificity Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4
Cross Reactivity Human, Mouse, Rat, Chicken
Applications FC, IHC
Immunogen O4 antibody was raised in Mouse using Bovine brain corpus callosum white matter as the immunogen

Images

Immunocytochemical staining of O4 antibody (10R-8060)

O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 ug/mL Human/Mouse/Rat/Chicken O4 antibody (10R-8060) for 3 hours at room temperature.

Specifications

Host Mouse
Clone O4
Isotype IgM
Form & Buffer Lyophilized from a 0.2 um filtered solution in PBS with Trehalose. Reconstitute at 0.5 mg/mL in sterile PBS

Usage & Assay Information

Usage Recommendations FC: 2.5 ug/10^6 cells, IHC: 110 ug/ml

Storage & Safety

Storage Store at -20 to -70 deg C

General Information

Biological Significance Oligodendrocytes and astrocytes are derived from common precursor sells, glioblasts. O-Antigens are sulfatides, which function as differentiation markers on the surface of oligodendrocytes of the central nervous system. O4 is formed postnatallyand is a marker for cell bodies and processes of oligodendrocytes types I and II (hairy eyeball type). During oligodendrocyte differentiation, O4 occurs in prooligodendrocytes, but not in O-2A-progenitor cells. O4 occurs from day 3 onwards in cell cultures of embryonic mouse brain.

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Images

  • Immunocytochemical staining of O4 antibody (10R-8060) | O4 in Differentiated Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 ug/mL Human/Mouse/Rat/Chicken O4 antibody (10R-8060) for 3 hours at room temperature.
  • Immunocytochemical staining of O4 antibody (10R-8060) | Oligodendrocyte Marker O4 in Rat Cortical Stem Cells. Oligodendrocyte Marker O4 was detected in immersion fixed 7 day differentiated rat cortical stem cells using 1 ug/m Human/Mouse/Rat/Chicken O4 antibody (10R-8060)for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue).

Availability: In stock

Price: $375.00
Size: 50 ug
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