OAT antibody (10R-1173)

Mouse monoclonal OAT antibody

Synonyms Monoclonal OAT antibody, Anti-OAT antibody, HOGA antibody, DKFZp781A11155 antibody, Ornithine aminotransferase antibody, ornithine aminotransferase precursor antibody, Ornithine oxo-acid aminotransferase antibody, ornithine aminotransferase (gyrate atrophy) antibody, ornithine aminotransferase (gyrate atrophy) antibody, ornithine aminotransferase precursor EC 2.6.1.13 antibody, Ornithine-oxo-acid aminotransferase. antibody, OATASE antibody, DKFZp781A11155 antibody, HOGA antibody, OATASE antibody, ornithine aminotransferase precursor antibody, Anti OAT antibody , ornithine aminotransferase precursor EC 2.6.1.13 antibody, mitochondrial precursor antibody, mitochondrial precursor antibody, Ornithine oxo-acid aminotransferase antibody, Ornithine-oxo-acid aminotransferase. antibody, OAT antibody, Ornithine aminotransferase antibody
Specificity Human
Cross Reactivity Human, Mouse
Applications ELISA, IF, WB
Immunogen OAT antibody was raised in mouse using recombinant human OAT (33-439aa) purified from E.coli as the immunogen.

Images

Western Blot analysis of OAT antibody (10R-1173)

Tissue lysates of mouse liver (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT23A2
Isotype IgG1 kappa
Source Anti-human OAT antibody, clone AT23A2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human OAT protein.
Method of Purification OAT antibody was purified by protein-G affinity chromatography
Form & Buffer Supplied as a liquid in PBS, pH 7.4, with 0.1% NaN3.
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations IF: 1:250-1:500, WB: 1:250-1:500

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance OAT is a 49-kDa nucleus-encoded protein imported into mitochondria to give the mature 48-kDa OAT polypeptide. It has been described in humans, animals, insects, plants and microorganisms. Especially OAT have sex-differential expression in the mouse kidney. OAT plays crucial physiological roles in amino acid metabolism. OAT shows a large structural and mechanistic similarity to other enzymes from the subgroup III of aminotransferases, which transfer an amino group from a carbon atom that does not carry a carboxyl function. OAT is essential for nitrogen recycling from arginine but not for the stress-induced proline accumulation.

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Images

  • Western Blot analysis of OAT antibody (10R-1173) | Tissue lysates of mouse liver (35 ug) were resolved by SDS-PAGE, transferred to nitrocellulose membrane and probed with anti-human OAT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $295.00
Size: 100 ul
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