OCT4 protein (His tag) (80R-2652)

Purified recombinant Human OCT4 protein (His tag)

Synonyms OTF-4 protein, OCT3 protein, OTF 4 protein, OCT 4 protein, OTF 3 protein, OCT-4 protein, POU domain protein, OTF-3 protein, OTF3 protein, OCT-4 protein, OCT 3 protein, OCT-3 protein, OCT 4, OCT4, OTF 4 protein, class 5 protein, MGC22487 protein, POU5F1 protein, OCT4 protein, OCT-4, OCT 4 protein, transcription factor 1 isoform 2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of OCT4 protein (His tag) (80R-2652)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-265 aa: MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQA DVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKP TLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAF PPVSVTTLGS PMHSN
Expression System E.coli
Method of Purification OCT4 protein was purified using Protein G affinity chromatography
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration 500 ug/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance POU domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3'). This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency. Recombinant human OCT4 protein, fused to His-tag at N-terminus, was expressed in E.coli.

References
Add a Paper

Wongtrakoongate P, Riddick G, Fucharoen S, Felsenfeld G2.

Association of the Long Non-coding RNA Steroid Receptor RNA Activator (SRA) with TrxG and PRC2 Complexes

PLoS Genet.

PMID: 26496121 DOI: 10.1371/journal.pgen.1005615

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of OCT4 protein (His tag) (80R-2652)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $395.00
Size: 100 ug
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