PAI1 protein (His tag) (80R-1029)
Purified recombinant Human PAI1 protein
|Synonyms||PAI 1 protein, Plasminogen activator inhibitor-1, PAI-1 protein, SERPINE1 protein, PAI1 protein, PAI-1, PAI 1, PAI1, PLANH1 protein, PLANH1 protein, Plasminogen activator inhibitor-1 protein|
Coomassie Blue stained SDS-PAGE of PAI1 protein (His tag) (80R-1029)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||24-402 amino acids: MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP|
|Purity||> 95% pure|
|Molecular Weight||45 kDa (400 aa)|
|Form & Buffer||Supplied as a liquid in 150mM Na2HPO4, pH 6.6, containing 0.5mM NaCl, 2mM Glutathione, and 0.01% Tween-80.|
Usage & Assay Information
|Bioactivity||Specific activity, the IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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