PAPSS1 protein (His tag) (80R-2132)

Recombinant human PAPSS1 protein (His tag)

Synonyms PAPSS1, PAPSS-1, PAPSS 1, PAPSS 1 protein, PAPSS-1 protein, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 protein, ATPSK1 protein, PAPSS protein, SK1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PAPSS1 protein (His tag) (80R-2132)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 24-624 amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA
Expression System E.coli
Purity > 85% pure
Molecular Weight 70.9 kDa (626aa)
Tag/Conjugate His tag
Form & Buffer Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl
Concentration 0.5 mg/ml

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of PAPSS1 protein (His tag) (80R-2132) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $365.00
Size: 100 ug
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