PCMT protein (His tag) (80R-1342)

Purified recombinant Human PCMT protein

Synonyms protein, , Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase protein, PCMT 1 protein, PIMT protein, Protein beta aspartate methyltransferase protein, L isoaspartyl/D aspartyl methyltransferase protein, Protein L isoaspartate (D aspartate) O methyltransferase protein, Protein L isoaspartyl/D aspartyl methyltransferase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PCMT protein (His tag) (80R-1342)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-227 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK
Expression System E.coli
Purity > 95% pure
Molecular Weight 28.8 kDa (263aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PCMT protein (His tag) (80R-1342) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
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