PCMT protein (His tag) (80R-1342)
Purified recombinant Human PCMT protein
|Synonyms||protein, , Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase protein, PCMT 1 protein, PIMT protein, Protein beta aspartate methyltransferase protein, L isoaspartyl/D aspartyl methyltransferase protein, Protein L isoaspartate (D aspartate) O methyltransferase protein, Protein L isoaspartyl/D aspartyl methyltransferase protein|
Coomassie Blue stained SDS-PAGE of PCMT protein (His tag) (80R-1342)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-227 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK|
|Purity||> 95% pure|
|Molecular Weight||28.8 kDa (263aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 0.1M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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