PDIA3 protein (His tag) (80R-1245)

Purified recombinant Human PDIA3 protein

Synonyms PDIA3 protein, Phospholipase C alpha protein, Protein disulfide-isomerase A3 58 kDa glucose regulated protein protein, GRP 57 protein, GRP 58 protein, HsT17083 protein, PDIA 3 protein, Glucose Regulated Protein 58 Kd protein, ER60 protein, Protein disulfide isomerase A3 protein, GRP58 protein, 58 kDa microsomal protein protein, ERp 57 protein, PI PLC protein, ER p57 protein, Protein disulfide isomerase family A member 3., PDIA3, PDIA-3 protein, P58 protein, PDIA 3, PDIA 3 protein, ERp61 protein, ERp60 protein, PI-PLC protein, ERp57 protein, Disulfide isomerase ER 60 protein, HsT17083 protein, GRP57 protein, PDIA-3
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PDIA3 protein (His tag) (80R-1245)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 25-505 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
Expression System E.coli
Purity > 95% pure
Molecular Weight 58.5 kDa (518aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 0.1 M NaCl and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final protein conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PDIA3 protein (His tag) (80R-1245) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $295.00
Size: 100 ug
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