PDK4 antibody (70R-2477)
Rabbit polyclonal PDK4 antibody raised against the N terminal of PDK4
Overview
Overview
| Synonyms | Polyclonal PDK4 antibody, Anti-PDK4 antibody, PDK 4, PDK 4 antibody, PDK-4 antibody, Pyruvate Dehydrogenase Kinase Isozyme 4 antibody, PDK-4, FLJ40832 antibody, PDK4 |
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| Specificity | PDK4 antibody was raised against the N terminal of PDK4 |
| Cross Reactivity | Human |
| Applications | IHC, WB |
| Immunogen | PDK4 antibody was raised using the N terminal of PDK4 corresponding to a region with amino acids MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACE |
| Assay Information | PDK4 Blocking Peptide, catalog no. 33R-6121, is also available for use as a blocking control in assays to test for specificity of this PDK4 antibody |
Images
Immunohistochemical staining using PDK4 antibody (70R-2477)
PDK4 antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Skeletal muscle cells (arrows) in Human Muscle. Magnification is at 400X
PDK4 antibody
Immunohistochemical staining using PDK4 antibody (70R-2477)
PDK4 antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml to stain Skeletal muscle cells (arrows) in Human Muscle. Magnification is at 400X
Western Blot analysis using PDK4 antibody (70R-2477)
PDK4 antibody (70R-2477) used at 1 ug/ml to detect target protein.
Specifications
| Host | Rabbit |
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| Method of Purification | Affinity purified |
| Molecular Weight | 46 kDa (MW of target protein) |
| Form & Buffer | Lyophilized powder. Add 50ul distilled water for a 1mg/ml concentration of PDK4 antibody in PBS |
| Concentration | 1 mg/ml |
Usage & Assay Information
| Usage Recommendations | WB: 1 ug/ml; IHC: 4-8 ug/ml |
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Storage & Safety
| Storage | Store at 2-8 deg C for short periods. For longer periods of storage, store at -20 deg C. Avoid repeat freeze-thaw cycles. |
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General Information
| Biological Significance | PDK4 is a member of the PDK/BCkDaK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. |
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