PDZK1 antibody (10R-8664)
Mouse monoclonal PDZK1 antibody
|Synonyms||Monoclonal PDZK1 antibody, Anti-PDZK1 antibody, Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody, CAP70 antibody, CLAMP antibody, NHERF-3 antibody, NHERF3 antibody, PDZD1 antibody|
|Immunogen||PDZK1 antibody was raised in Mouse using recombinant human PDZK1 (1-519aa) purified from E. coli as the immunogen|
Western Blot analysis using PDZK1 antibody (10R-8664)
The cell lysates of HeLa (40 ug) and 293T (40 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PDZK1 antibody (1:1000).
|Method of Purification||PDZK1 antibody was purified by protein-A affinity chromatography|
|Form & Buffer||Supplied in liquid form in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol and 0.02% Sodium Azide|
Usage & Assay Information
|Usage Recommendations||ELISA: 1:1000|
Storage & Safety
|Storage||Can be stored at 4 deg C short term. For long term storage, aliquot and store at -20 deg C. Avoid repeated freezing and thawing cycles.|
|Biological Significance||PDZK1 is PDZ domain-containing scaffolding protein with a 519 amino acid protein. PDZK1 mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. This protein is expressed in the kidney, pancreas, liver, gastrointestinal tract, and adrenal cortex. In situ hybridization experiments showed that the expression of PDZK1 was limited to epithelial cells. In addition, PDZK1 was overexpressed in selected tumors of epithelial origin.|
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