PECI protein (His tag) (80R-1368)
Purified recombinant Human PECI protein
|Synonyms||acyl Coenzyme A binding domain containing 2 protein, DBI related protein 1 protein, ACBD2 protein, Peroxisomal 3 protein, Hepatocellular carcinoma associated protein 88 protein, Dodecenoyl CoA isomerase protein, DRS1 protein, HCA 88 protein, D3 D2 enoyl CoA isomerase protein, Delta(3) delta(2) enoyl CoA isomerase protein, DRS 1 protein, Peroxisomal D3 D2 enoyl CoA isomerase protein, 2-trans-enoyl-CoA isomerase protein, HCA88 protein, Renal carcinoma protein NY REN 1|
Coomassie Blue stained SDS-PAGE of PECI protein (His tag) (80R-1368)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-364 amino acids: MGSSHHHHHH SSGLVPRGSH MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL|
|Purity||> 95% pure|
|Molecular Weight||42.3 kDa (384aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the ?-oxidation of unsaturated fatty acids. Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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