PEF1 protein (His tag) (80R-1663)
Purified recombinant Human PEF1 protein
|Synonyms||PEF-1 protein, ABP32 protein, PEF 1, PEF 1 protein, PEF1, PEF1A, PEF-1, Penta-EF-hand domain containing 1 protein|
Coomassie Blue stained SDS-PAGE of PEF1 protein (His tag) (80R-1663)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-284 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMASY PYRQGCPGAA GQAPGAPPGS YYPGPPNSGG QYGSGLPPGG GYGGPAPGGP YGPPAGGGPY GHPNPGMFPS GTPGGPYGGA APGGPYGQPP PSSYGAQQPG LYGQGGAPPN VDPEAYSWFQ SVDSDHSGYI SMKELKQALV NCNWSSFNDE TCLMMINMFD KTKSGRIDVY GFSALWKFIQ QWKNLFQQYD RDRSGSISYT ELQQALSQMG YNLSPQFTQL LVSRYCPRSA NPAMQLDRFI QVCTQLQVLT EAFREKDTAV QGNIRLSFED FVTMTASRML|
|Purity||> 85% pure|
|Molecular Weight||34.5 kDa (320aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 50% glycerol and 0.2M NaCl.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||Penta-EF-hand domain containing 1, also known as PEF1, belongs to the penta-EF-hand protein family, which shares similarities to the apoptosis linked gene ALG-2. PEF1 heterodimerizes with PDCD6/ALG2 and dissociates from PDCD6/ALG2 in the presence of Ca (2+). Also, PEF1 proteins bind calcium and participate in a variety of calcium-dependent processes in vertebrates. Recombinant human PEF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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