PFKM antibody (10R-8651)

Mouse monoclonal PFKM antibody

Synonyms Monoclonal PFKM antibody, Anti-PFKM antibody, GSD7 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, 6-Phosphofructokinase antibody, muscle type 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, MGC8699 antibody, PFKL antibody, PFKM antibody, PFKP antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody, Phosphofructokinase M antibody, Phosphofructokinase antibody, muscle antibody, Phosphofructokinase antibody, muscle type antibody, Phosphofructokinase antibody, polypeptide X antibody, Phosphohexokinase.
Applications ELISA, WB
Immunogen PFKM antibody was raised in Mouse using a recombinant human PFKM protein as the immunogen

Images

Western Blot analysis using PFKM antibody (10R-8651)

The HepG2 cell lysate (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PFKM antibody (1:500 - 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Host Mouse
Clone AT2F11
Isotype IgG2a kappa
Method of Purification PFKM antibody was purified by protein A affinity chromatography
Form & Buffer Supplied in liquid form in PBS, pH 7.4, containing 10% Glycerol, 0.01% Sodium Azide
Concentration 1 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:2000, WB: 1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage, for long term storage aliquot and store at -20 deg C, avoid repeated freeze thaw cycles.

General Information

Biological Significance PFKM(phosphofructokinase, muscle) is a regulatory glycolytic enzyme that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.

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Images

  • Western Blot analysis using PFKM antibody (10R-8651) | The HepG2 cell lysate (30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with PFKM antibody (1:500 - 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Availability: In stock

Price: $175.00
Size: 50 ul
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