PGA5 antibody (10R-1871)

Mouse monoclonal PGA5 antibody

Synonyms Monoclonal PGA5 antibody, Anti-PGA5 antibody, Pepsinogen 5 group I antibody, Pepsinogen A antibody, PGA5, PGA 5, PGA-5, PGA 5 antibody, PGA-5 antibody
Cross Reactivity Human
Applications ELISA, IHC, WB
Immunogen PGA5 antibody was raised in Mouse using a purified recombinant fragment of human PGA5 expressed in E. coli as the immunogen.

Images

Immunohistochemical staining using PGA5 antibody (10R-1871)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 antibody with DAB staining.

Specifications

Host Mouse
Clone 2C1
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, IHC: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor,pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates.

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Images

  • Immunohistochemical staining using PGA5 antibody (10R-1871) | Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using PGA5 antibody with DAB staining.
  • Western Blot analysis using PGA5 antibody (10R-1871) | Western Blot showing PGA5 antibody used against HepG2 (1) and SMMC-7721 (2) cell lysate.

Availability: In stock

Price: $345.00
Size: 100 ul
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