PGLS protein (His tag) (80R-1520)

Purified recombinant Human PGLS protein

Synonyms 6PGL, 6-phosphogluconolactonase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PGLS protein (His tag) (80R-1520)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-258 amino acids: MGSSHHHHHH SSGLVPRGSH MAAPAPGLIS VFSSSQELGA ALAQLVAQRA ACCLAGARAR FALGLSGGSL VSMLARELPA AVAPAGPASL ARWTLGFCDE RLVPFDHAES TYGLYRTHLL SRLPIPESQV ITINPELPVE EAAEDYAKKL RQAFQGDSIP VFDLLILGVG PDGHTCSLFP DHPLLQEREK IVAPISDSPK PPPQRVTLTL PVLNAARTVI FVATGEGKAA VLKRILEDQE ENPLPAALVQ PHTGKLCWFL DEAAARLLTV PFEKHSTL
Expression System E.coli
Purity > 95% pure
Molecular Weight 29.7 kDa (278aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 10% glycerol and 100mM NaCl.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance 6-phosphogluconolactonase, also known as PGLS, is an enzyme in the second step pentose phosphate pathway, which is essential for the synthesis of nucleotide sugars and NADPH, the main source of reducing power. It converts 6-phosphogluconolactone to 6-phosphogluconate. Recombinant human PGLS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PGLS protein (His tag) (80R-1520) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 50 ug
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