phospho-Phospholamban antibody (20R-P121a)

Rabbit polyclonal phospho-Phospholamban antibody

Synonyms Polyclonal phospho-Phospholamban antibody, Anti-phospho-Phospholamban antibody, pPhospholamban antibody, Phosphorylated Phospholamban antibody
Specificity human
Cross Reactivity Bovine, dog, mouse, rat
Applications WB
Immunogen Phospho-Phospholamban antibody was raised in rabbit using a synthetic peptide conjugated to KLH, corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) as the immunogen.

Images

Western blot analysis using phospho-Phospholamban antibody (20R-P121a)

Western blot analysis of Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), probed with anti-phospho-phospholamban (0.5ug/ml)

Specifications

Host Rabbit
Isotype IgG
Method of Purification phospho-Phospholamban antibody was purified by affinity chromatography.
Form & Buffer 100mM Tris-glycine, pH 7.4, with 150mM NaCl, 0.05% NaN3 and 30% glycerol.
Concentration Batch dependent - please inquire should you have specific requirements

Usage & Assay Information

Usage Recommendations Optimal conditions to be determined by end user

Storage & Safety

Storage Aliquot and store at -20 deg C. Avoid repeated Freeze/Thaw cycles
Biohazard Information This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling.

General Information

Biological Significance Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

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Images

  • Western blot analysis using phospho-Phospholamban antibody (20R-P121a) | Western blot analysis of Bovine cardiac preparation, treated with PKA (lane 1) or untreated (lane 2), probed with anti-phospho-phospholamban (0.5ug/ml)

Availability: In stock

Price: $555.00
Size: 100 ug
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