Phosphoserine phosphatase protein (30R-1072)
Purified recombinant Human Phosphoserine phosphatase protein
|Synonyms||EC 188.8.131.52 protein, PSPase protein, PSP protein, L 3 phosphoserine phosphatase protein, , Phosphoserine phosphatase deficiency protein, included protein, Phosphoserine phosphatase Human protein, L-3-phosphoserine phosphatase protein, Phosphoserine phosphatase protein, O phosphoserine phosphohydrolase protein, O-phosphoserine phosphohydrolase protein, PSPH protein|
Coomassie Blue stained SDS-PAGE of Phosphoserine phosphatase protein (30R-1072)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||MVSHSELRKL FYSADAVCFD VDSTVIREEG IDELAKICGV EDAVSEMTRR AMGGAVPFKA ALTERLALIQ PSREQVQRLI AEQPPHLTPG IRELVSRLQE RNVQVFLISG GFRSIVEHVA SKLNIPATNV FANRLKFYFN GEYAGFDETQ PTAESGGKGK VIKLLKEKFH FKKIIMIGDG ATDMEACPPA DAFIGFGGNV IRQQVKDNAK WYITDFVELL GELEE|
|Purity||> 95% pure|
|Molecular Weight||25 kDa (225 aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 10mM Tris-HCl, pH 7.5, containing 50mM NaCl, 2mM DTT, 1mM MnCl, and 20% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Endotoxin Levels||< 1.0 EU per ug of protein (determined by LAL method)|
|Biological Significance||Protein phosphatase 2C (PP2Calpha) is a Mn +/- or Mg +/-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2Calpha(amino acids 1-382) was cloned into an E. coli expression vector. PP2Calpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.|
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