PHPT1 protein (His tag) (80R-1513)
Purified recombinant Human PHPT1 protein
|Synonyms||14 kDa phosphohistidine phosphatase protein, bA216L13.10 protein, PHP14 protein, CGI-202 protein, PHPT-1 protein, PHPT 1, RP11-216L13.10, PHPT 1 protein, PHPT-1, HSPC141 protein, DKFZp564M173 protein, PHPT1|
Coomassie Blue stained SDS-PAGE of PHPT1 protein (His tag) (80R-1513)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-125 amino acids: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY|
|Purity||> 95% pure|
|Molecular Weight||15.9 kDa (145aa) confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.2M NaCl, 2mM DTT and 10% glycerol.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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