PIP4K2B protein (His tag) (80R-2646)
Purified recombinant Human PIP4K2B protein (His tag)
|Synonyms||PI5P-4KB protein, PIP5KII beta protein, PIP5KIIB protein, PIP5K 2B protein, PIP5KII-beta protein, PIP5K-IIB protein, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta protein, PIPK2B-4 protein, PIPK2B 4 protein, PIPK2B 4, PIP5K-2B protein, PIPK2B-4, PI5P 4KB protein, PIP5KIIbeta protein, PIP5K IIB protein, PIP4K2B, PI5P4KB protein, PIP5K2B protein|
SDS-PAGE analysis of PIP4K2B protein (His tag) (80R-2646)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-416 aa: MGSSHHHHHH SSGLVPRGSH MGSMSSNCTS TTAVAVAPLS ASKTKTKKKH FVCQKVKLFR ASEPILSVLM WGVNHTINEL SNVPVPVMLM PDDFKAYSKI KVDNHLFNKE NLPSRFKFKE YCPMVFRNLR ERFGIDDQDY QNSVTRSAPI NSDSQGRCGT RFLTTYDRRF VIKTVSSEDV AEMHNILKKY HQFIVECHGN TLLPQFLGMY RLTVDGVETY MVVTRNVFSH RLTVHRKYDL KGSTVAREAS DKEKAKDLPT FKDNDFLNEG QKLHVGEESK KNFLEKLKRD VEFLAQLKIM DYSLLVGIHD VDRAEQEEME VEERAEDEEC ENDGVGGNLL CSYGTPPDSP GNLLSFPRFF GPGEFDPSVD VYAMKSHESS PKKEVYFMAI IDILTPYDTK KKAAHAAKTV KHGAGAEIST VNPEQYSKRF NEFMSNILT|
|Method of Purification||PIP4K2B protein was purified using Protein G affinity chromatography|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PIP4K2B catalyzes the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. It is a member of the phosphatidylinositol-4-phosphate 5-kinase family. The amino acid sequence of PIP4K2B does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, PIP4K2B interacts with p55 TNF receptor. Recombinant human PIP4K2B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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