PIRH2 antibody (10R-1887)

Mouse monoclonal PIRH2 antibody

Synonyms Monoclonal PIRH2 antibody, Anti-PIRH2 antibody, PIRH-2 antibody, PIRH 2 antibody, P53 induced RING-H2 protein antibody, RCHY1 antibody, PIRH2, PIRH 2, PIRH-2, PIRH 2 antibody, PIRH-2 antibody
Cross Reactivity Human, Rat
Applications ELISA, FC, IF, WB
Immunogen PIRH2 antibody was raised in Mouse using a purified recombinant fragment of human PIRH2 expressed in E. coli as the immunogen.

Images

Flow Cytometric assay using Western Blot analysis using PIRH2 antibody (10R-1887) (PIRH2 antibody)

FC analysis of PC-12 cells using anti-Pirh2 antibody (blue) and negative control (red).

Specifications

Host Mouse
Clone 1H10
Isotype IgG1
Form & Buffer Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration 1.0 mg/ml

Usage & Assay Information

Usage Recommendations ELISA: 1:10,000, FC: 1:200-1:400, IF: 1:200-1:1000, WB: 1:500-1:2000

Storage & Safety

Storage Store at 4 deg C for short term storage. Store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PIRH2 forms dimers through its N- and C-terminus in cells. PIRH2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The PIRH2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. PIRH2 expression decreases the level of p53, and a decrease of endogenous PIRH2 expression increases p53 levels. PIRH2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Flow Cytometric assay using Western Blot analysis using PIRH2 antibody (10R-1887) (PIRH2 antibody) | FC analysis of PC-12 cells using anti-Pirh2 antibody (blue) and negative control (red).
  • Western Blot analysis using PIRH2 antibody (10R-1887) | Western Blot showing Pirh2 antibody used against Hela (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate.
  • Immunohistochemical staining using PIRH2 antibody (10R-1887) | Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using anti-Pirh2 antibody
  • Immunofluorescent staining using Western Blot analysis using PIRH2 antibody (10R-1887) (PIRH2 antibody) | Immunofluorescence analysis of Hela cells using Pirh2 antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Availability: In stock

Price: $345.00
Size: 100 ul
OR
Shipping
View Our Distributors