PLK1 antibody (10R-8477)

Mouse monoclonal PLK1 antibody

Synonyms Monoclonal PLK1 antibody, Anti-PLK1 antibody, PLK1 antibody, polo-like kinase 1 antibody, BOS_22765 antibody, cell cycle regulated protein kinase antibody, EGK_12605 antibody, ik:tdsubc_2d1 antibody, NV_30481 antibody, NV50027 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, polo (Drosophia)-like kinase antibody, polo like kinase antibody, polo-like kinase 1 (Drosophila) antibody, polo-like kinase homolog antibody, polo-like protein kinase antibody, RCJMB04_20p15 antibody, serine/threonine-protein kinase 13 antibody, serine/threonine-protein kinase PLK1 antibody, STPK13 antibody, xx:tdsubc_2d1 antibody
Cross Reactivity Human, Mouse, Rat
Applications ELISA, IP, WB
Immunogen PLK1 antibody was raised in Mouse using a recombinant human protein purified from E.coli (GST/His-Plk1) as the immunogen

Images

Western Blot analysis using PLK1 antibody (10R-8477)

Specifications

Host Mouse
Clone 18D4
Isotype IgG1 kappa
Method of Purification PLK1 antibody was purified by ammonium sulfate precipitation
Form & Buffer Supplied in liquid form in HEPES buffer with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol

Usage & Assay Information

Usage Recommendations WB: 1:1000, IP: 1 ul

Storage & Safety

Storage Aliquot and store for 1 year at -20 deg C, avoid freeze/thaw cycles

General Information

Biological Significance Polo-like kinases (Plks) are important regulators of the cell cycle. Plk1, the most studied Plk, has been implicated in regulating centrosome maturation, mitotic entry, sister chromatid cohesion, the anaphase-promoting complex/cyclosome (APC/C), and cytokinesis. Sister chromatid separation and the subsequent formation of two genetically identical daughter cells depend on the symmetrical attachment of all chromosomes to the mitotic spindle, a process called chromosome biorientation. Several mitosis-specific protein kinases have been implicated in bipolar spindle assembly and chromosome biorientation. For example, Cdk1 (cyclin-dependent kinase 1) is known to phosphorylate Eg5, a kinesin that is required for the migration of centrosomes and is thus needed for the formation of bipolar spindles.At the onset of mitosis, Plks contribute to the activation of Cdk1-cyclinB, and they are also required for the inactivation of Cdk1 and exit from mitosis. Plks are important regulators of the APC/C, a key component of the ubiquitin-dependent proteolytic degradation pathway.

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Images

  • Western Blot analysis using PLK1 antibody (10R-8477) |

Availability: In stock

Price: $295.00
Size: 100 ul
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