PNP protein (His tag) (80R-1547)

Purified recombinant Human PNP protein

Synonyms PUNP, PRO1837 protein, NP protein, Purine nucleoside phosphorylase protein, Inosine phosphorylase protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PNP protein (His tag) (80R-1547)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-289 amino acids: MGSSHHHHHH SSGLVPRGSH MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS
Expression System E.coli
Purity > 90% pure
Molecular Weight 34.2 kDa (309aa) confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 0.1M NaCl, 2mM DTT and 10% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2?-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

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Images

  • Coomassie Blue stained SDS-PAGE of PNP protein (His tag) (80R-1547) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $255.00
Size: 100 ug
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