POFUT1 protein (His tag) (80R-2617)

Purified recombinant Human POFUT1 protein (His tag)

Synonyms O-FUT protein, POFUT-1, O-Fuc-T protein, O FUT protein, FUT 12 protein, OFUT protein, FUT-12 protein, O-FucT-1 protein, POFUT 1, FUT12 protein, POFUT1, POFUT 1 protein, POFUT-1 protein, POFUT 1 protein, POFUT1 protein, GDP-fucose protein O-fucosyltransferase 1 protein, POFUT-1 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

SDS-PAGE analysis of POFUT1 protein (His tag) (80R-2617)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 27-388 aa: MGSSHHHHHH SSGLVPRGSH MGSGSWDPAG YLLYCPCMGR FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL EPLQAYHRVI SLEDFMEKLA PTHWPPEKRV AYCFEVAAQR SPDKKTCPMK EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YREQWSQRFS PKEHPVLALP GAPAQFPVLE EHRPLQKYMV WSDEMVKTGE AQIHAHLVRP YVGIHLRIGS DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TAAPLTMTMC LPDLKEIQRA VKLWVRSLDA QSVYVATDSE SYVPELQQLF KGKVKVVSLK PEVAQVDLYI LGQADHFIGN CVSSFTAFVK RERDLQGRPS SFFGMDRPPK LRDEF
Expression System E.coli
Method of Purification POFUT1 protein was purified using Protein G affinity chromatography
Tag/Conjugate His tag
Form & Buffer Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance GDP-fucose protein O-fucosyltransferase 1, also known as POUFUT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFUT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFUT1 plays an important role in Notch signaling, as Notch ligands can serve as POFUT1 substrates. Recombinant human POFUT1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

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Images

  • <div><font face=Calibri size=2>SDS-PAGE analysis of POFUT1 protein (His tag) (80R-2617)</font></div>     | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 100 ug
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