POLR2G antibody (10R-1427)

Mouse monoclonal POLR2G antibody

Synonyms Monoclonal POLR2G antibody, Anti-POLR2G antibody, POLR2G, hsRPB7 antibody, POLRG 2 antibody, POLRG 2, hRPB19 antibody, RNA polymerase II subunit B7 antibody, MGC138369 antibody, MGC138367 antibody, POLRG-2, POLRG-2 antibody, RPB7 antibody
Specificity Human POLR2G
Cross Reactivity Human, Mouse, Rat. Other species not studied
Applications Dot Blot, FC, WB
Immunogen POLR2G antibody was raised in mouse using recombinant Human Polymerase (Rna) Ii (Dna Directed) Polypeptide G (Polr2G)

Images

Flow Cytometric assay using POLR2G antibody (10R-1427)

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.

Specifications

Host Mouse
Clone 2781C2a
Isotype IgG1
Method of Purification POLR2G antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer POLR2G antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, FC: 0.5-2 ug/sample

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3A protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.

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Images

  • Flow Cytometric assay using POLR2G antibody (10R-1427) | HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG.
  • Western Blot analysis of immunized recombinant protein using POLR2G antibody (10R-1427)

Availability: In stock

Price: $190.00
Size: 50 ug
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