POLR2J2 protein (His tag) (80R-2656)
Purified recombinant Human POLR2J2 protein (His tag)
|Synonyms||POLRJ-2 protein, POLRJ 2, RPB11b1 protein, POLRJ-2, DNA-directed RNA polymerase II subunit RPB11-b1 protein, POLR2J2, HRPB11B protein, POLRJ 2 protein|
SDS-PAGE analysis of POLR2J2 protein (His tag) (80R-2656)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-115 aa: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP KACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP|
|Method of Purification||POLR2J2 protein was purified using Protein G affinity chromatography|
|Form & Buffer||Supplied in liquid form in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT, 250mM Imidazole|
Storage & Safety
|Storage||Store at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
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