POU2F1 antibody (10R-1201)

Mouse monoclonal POU2F1 antibody

Synonyms Monoclonal POU2F1 antibody, Anti-POU2F1 antibody, POUF1-2, POUF1 2 antibody, POU2F1, POUF1 2, OTF1 antibody, OCT1 antibody, POU domain class 2 transcription factor 1 antibody, POUF1-2 antibody
Specificity Human POU2F1
Cross Reactivity Human. Other species not studied
Applications Dot Blot, ICC, WB
Immunogen POU2F1 antibody was raised in mouse using recombinant Pou Domain, Class 2, Transcription Factor 1

Images

Immunofluorescent staining using POU2F1 antibody (10R-1201)

Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-POU2F1 antibody.

Specifications

Host Mouse
Clone POU5I097
Isotype IgG1
Method of Purification POU2F1 antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer POU2F1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations WB: 0.2-2 ug/ml, ICC: 2-100 ug/ml

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR .

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Images

  • Immunofluorescent staining using POU2F1 antibody (10R-1201) | Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-POU2F1 antibody.
  • Western Blot analysis of immunized recombinant protein using POU2F1 antibody (10R-1201)

Availability: In stock

Price: $190.00
Size: 50 ug
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