PPIE protein (His tag) (80R-1080)

Purified recombinant Human PPIE protein

Synonyms Peptidylprolyl isomerase E isoform 1 Cyclophilin 33 protein, , EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E protein, PPIase E protein, PPIE protein, Rotamase E protein, CYP33 protein, peptidylprolyl isomerase E (cyclophilin E) protein, Cyclophilin E protein, CYP-33 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1uM suc-AAFP-pNA). 2. Add recombinant Cyclophilin E protein with various concentrations (1ug, 2ug, 5ug) in assay buffer. 3. Mix by inversion and equilibrate to 1C and monitor the A410nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 uM and mix immediately. 5. Record the increase in A410 nm for 20 minutes

Images

Coomassie Blue stained SDS-PAGE of PPIE protein (His tag) (80R-1080)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-301 amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV
Expression System E.coli
Purity > 95% pure
Molecular Weight 37.5 kDa (337 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris, pH 8.0.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity is > 210 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 1 DEG C in Tris-Hcl pH 8.0 using chymotrypsin.

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE. Recombinant human PPIE was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PPIE protein (His tag) (80R-1080) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $340.00
Size: 100 ug
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