PPM1D antibody (10R-1650)

Mouse monoclonal PPM1D antibody

Synonyms Monoclonal PPM1D antibody, Anti-PPM1D antibody, PPMD 1 antibody, WIP1 antibody, PPM1D, Protein phosphatase 1D antibody, PPMD-1, PPMD-1 antibody, PPMD 1, PP2C-DELTA antibody
Specificity Human PPM1D
Cross Reactivity Human. Other species not studied
Applications Dot Blot
Immunogen PPM1D antibody was raised in mouse using recombinant Human Protein Phosphatase 1D Magnesiumdependent, Delta Isoform (Ppm1D)

Images

Western Blot analysis of immunized recombinant protein using PPM1D antibody (10R-1650)

Left: PPM1D staining. Right: Coomassie Blue staining of immunized recombinant protein.

Specifications

Host Mouse
Clone 2804D1a
Isotype IgG1
Method of Purification PPM1D antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Form & Buffer PPM1D antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3 ).
Concentration 100 ug/ml

Usage & Assay Information

Usage Recommendations To be optimized by the end user.

Storage & Safety

Storage Store at 2-8 deg C for up to one year. We recommend long term storage at -20 deg C. Avoid repeated freezing and thawing.
Biohazard Information This antibody solution contains sodium azide (NaN ) as a preservative. There is a potential hazard that NaN reacts with copper or lead to produce an explosive compound. For safe disposal, the vial has to be washed thoroughly with water. It is strongly adv

General Information

Biological Significance The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Western Blot analysis of immunized recombinant protein using PPM1D antibody (10R-1650) | Left: PPM1D staining. Right: Coomassie Blue staining of immunized recombinant protein.

Availability: In stock

Price: $190.00
Size: 50 ug
OR
Shipping
View Our Distributors