PPM1G protein (His tag) (80R-1052)

Purified recombinant Human PPM1G protein

Synonyms MGC1675 protein, PP2Cgamma protein, PPP2CG protein, Protein phosphatase 1G protein, PPM1G protein, Protein phosphatase 2C gamma isoform protein, PP2C gamma protein, PPMG 1, PPMG-1 protein, PPMG-1, MGC2870 protein, Protein phosphatase 2 catalytic subunit gamma isoform protein, Protein phosphatase 1G 'PP2C protein, PP2CG protein, PPM1G protein, PPMG 1 protein, Protein phosphatase 1C protein, Protein phosphatase magnesium dependent 1 gamma., PPM1G
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PPM1G protein (His tag) (80R-1052)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 317-546 amino acids: MGSSHHHHHH SSGLVPRGSH MEGKEEPGSD SGTTAVVALI RGKQLIVANA GDSRCVVSEA GKALDMSYDH KPEDEVELAR IKNAGGKVTM DGRVNGGLNL SRAIGDHFYK RNKNLPPEEQ MISALPDIKV LTLTDDHEFM VIACDGIWNV MSSQEVVDFI QSKISQRDEN GELRLLSSIV EELLDQCLAP DTSGDGTGCD NMTCIIICFK PRNTAELQPE SGKRKLEEVL STEGAEENGN SDKKKKAKRD
Expression System E.coli
Purity > 95% pure
Molecular Weight 27 kDa (250 aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 25mM Tris, pH 7.5, containing 1mM DTT, 1mM EDTA, 2mM beta-mercaptoethanol, and 20% glycerol.
Concentration 1 mg/ml

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Recombinant human PPM1G, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

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Images

  • Coomassie Blue stained SDS-PAGE of PPM1G protein (His tag) (80R-1052) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $180.00
Size: 100 ug
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