PPP1CA protein (His tag) (80R-1935)

Recombinant human PPP1CA protein

Synonyms PP-1A protein, PPPCA 1 protein, PPPCA 1, PPP1CA, PPP1A protein, PPPCA-1, PPPCA-1 protein, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit protein, PP1alpha protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE
Assay Information 1. Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris(pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2. Pre-warm reaction mix in heat block at 37C. 3. Add recombinant PPP1CA solution with various concentrations (2ug, 3ug) in 200ul reaction buffer. 4. Mix by inversion and incubate at 37C for 10minutes. 5. Stop the reaction by adding the 800ul of 0.1N NaOH. 6. Record the absorbance at 405nm

Images

Coomassie Blue stained SDS-PAGE of PPP1CA protein (His tag) (80R-1935)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 1-330 amino acids: MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
Expression System E.coli
Purity > 80% pure
Molecular Weight 39.7 kDa (350aa), confirmed by MALDI-TOF
Tag/Conjugate His tag
Form & Buffer Liquid. 50mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT, 0.1mM PMSF, 1mM MnCl2
Concentration 0.25 mg/ml

Usage & Assay Information

Usage Recommendations PPP1CA protein (His tag) has been used in SDS PAGE, and may be suitable for use in other assays, to be determined by the end user.
Bioactivity Specific activity: >3,000 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

Storage & Safety

Storage Can be stored at 4 deg C short term (1-2 weeks). For long term storage, aliquot and store at -20 deg C or -70 deg C. Avoid repeated freezing and thawing cycles.

General Information

Biological Significance PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Recombinant human PPP1CA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

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Images

  • Coomassie Blue stained SDS-PAGE of PPP1CA protein (His tag) (80R-1935) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $270.00
Size: 10 ug
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