PPP3R2 protein (His tag) (80R-1747)
Purified recombinant Human PPP3R2 protein
|Synonyms||Protein phosphatase 3 protein, PPPR2 3 protein, PPPR2-3, PPP3R2, regulatory subunit B type 2 protein, PPPR2-3 protein, PPP3RL protein, PPPR2 3|
Coomassie Blue stained SDS-PAGE of PPP3R2 protein (His tag) (80R-1747)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||1-173 amino acids: MGSSHHHHHH SSGLVPRGSH MSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFS IYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV|
|Purity||> 95% pure|
|Molecular Weight||22 kDa (193aa), confirmed by MALDI-TOF|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol and 1mM DTT.|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.|
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