PRDX4 protein (His tag) (80R-1280)
Purified recombinant Human PRDX4 protein
|Synonyms||PRX 4 protein, AOE372 protein, Peroxiredoxin4 protein, AOE37-2 protein, TRANK., Thioredoxin peroxidase protein, EC 18.104.22.168 protein, Thioredoxin dependent peroxide reductase A0372 protein, Prx IV protein, peroxiredoxin 4 Antioxidant enzyme 372 protein, Thioredoxin peroxidase AO372 protein, PRDX 4 protein, PRDX4 protein, PRDX-4, PRDX 4, PrxIV protein, PRX-4 protein, Antioxidant enzyme AOE372 protein, PRX4 protein, Thioredoxin Peroxidase (Antioxidant Enzyme) protein, PRDX4, PRDX 4 protein, PRDX-4 protein, AOE37 2 protein|
Coomassie Blue stained SDS-PAGE of PRDX4 protein (His tag) (80R-1280)
Figure annotation denotes ug of protein loaded and % gel used.
|Residues||38-271 amino acids: MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN|
|Purity||> 90% pure|
|Molecular Weight||28.8 kDa (255aa), confirmed by MALDI-TOF.|
|Form & Buffer||Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.|
Usage & Assay Information
|Bioactivity||Specific activity: approximately 230-310 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide|
Storage & Safety
|Storage||Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.|
|Biological Significance||PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.|
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