PRDX4 protein (His tag) (80R-1280)

Purified recombinant Human PRDX4 protein

Synonyms PRX 4 protein, AOE372 protein, Peroxiredoxin4 protein, AOE37-2 protein, TRANK., Thioredoxin peroxidase protein, EC 1.11.1.15 protein, Thioredoxin dependent peroxide reductase A0372 protein, Prx IV protein, peroxiredoxin 4 Antioxidant enzyme 372 protein, Thioredoxin peroxidase AO372 protein, PRDX 4 protein, PRDX4 protein, PRDX-4, PRDX 4, PrxIV protein, PRX-4 protein, Antioxidant enzyme AOE372 protein, PRX4 protein, Thioredoxin Peroxidase (Antioxidant Enzyme) protein, PRDX4, PRDX 4 protein, PRDX-4 protein, AOE37 2 protein
Species Human
Protein Type Recombinant
Applications SDS-PAGE

Images

Coomassie Blue stained SDS-PAGE of PRDX4 protein (His tag) (80R-1280)

Figure annotation denotes ug of protein loaded and % gel used.

Specifications

Residues 38-271 amino acids: MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN
Expression System E.coli
Purity > 90% pure
Molecular Weight 28.8 kDa (255aa), confirmed by MALDI-TOF.
Tag/Conjugate His tag
Form & Buffer Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 10% glycerol.
Concentration 1 mg/ml

Usage & Assay Information

Bioactivity Specific activity: approximately 230-310 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide

Storage & Safety

Storage Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

General Information

Biological Significance PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

References
Add a Paper

Sorry, but there are no references currently for this product.

Reviews

You need to be logged in to write a review. Please login here

Sorry there are currently no reviews for this product

Images

  • Coomassie Blue stained SDS-PAGE of PRDX4 protein (His tag) (80R-1280) | Figure annotation denotes ug of protein loaded and % gel used.

Availability: In stock

Price: $220.00
Size: 100 ug
OR
Shipping
View Our Distributors